Saina Nehwal's husband Parupalli Kashyap, who is a badminton player by profession as well, also lashed out at the umpire following the narrow defeat.
Saina Nehwal lost 21-15 25-27 12-21 against Mia Blichfeldt, seeded 12th, in a marathon women's singles match.
Saina and Sindhu could face-off at the semifinals if they get past the initial rounds of the tournament.
In the absence of PV Sindhu, who withdrew in the last minute from the event, Saina's ouster marks the end of India's campaign in the women's singles.
Nehwal, who withdrew from Indonesia Open and Japan Open due to injuries, registered a straight-game win over Phittayaporn Chaiwan of Thailand.
Among the men's singles shuttlers, Kidambi Srikanth and Sameer Verma managed to hold on to their 10th and 13th spots in the latest rankings issued on Tuesday.
PV Sindhu, who reached the final in Indonesia Open but lost in the Japan Open quarterfinals last week, will not take the court in the Thailand Open main draw.
The USD 750,000 tournament will also see the return of Saina Nehwal, who had missed the Indonesia Open Super 1000 event due to fitness issues
Saina Nehwal, as well as Sameer Verma skipped the ongoing BWF World Tour Super 1000 in Indonesia due to injuries.
Parineeti Chopra is busy going through rigorous training sessions for Saina Nehwal biopic.
India first will look to quell the challenge of Malaysia on Monday before taking on the mighty 10-time champions China the next day.
The 29-year-old Olympic bronze medallist and world number nine lost 16-21, 23-21, 4-21 to her 19-year-old opponent in the women's singles match that lasted one hour and seven minutes.
Nehwal, seeded second, will begin her quest for the second title this year when she will take on China's Wang Zhiyi in the first round of the women's singles.
Much was expected from fourth seed Sindhu but the Rio Olympic silver medallist was stunned by an unseeded local girl Cai Yanyan.
Sindhu looked in command from the beginning and registered a thumping 21-14, 21-7 victory in just 28 minutes against Japan's Takahashi Sayaka.
PV Sindhu defeated world No.18 Cai Yanyan of China 21-13, 17-21, 21-14 to set up a meeting with former world champion Nozomi Okuhara.
Star Indian shuttlers P V Sindhu and Saina Nehwal sailed into the second round of the Singapore Open with straight-game wins over their respective rivals in the women's singles event here Wednesday.
Parineeti Chopra recently shared a post adoring baby Izzu, the son of Sania Mirza. The post went viral when Parineeti Chopra is seen happily posing with baby Izhaan.
Last week, Saina, who had reached the quarter-finals of the All England Championship, had broken the news that she was advised to get admitted to get treated for her illness.
Tendulkar wished Saina a quick recovery as a few days back the badminton star revealed that she is being diagnosed with acute gastroenteritis with mild pancreatitis.
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