Portuguese health authorities said on Monday that one of those who tested positive at the Lisbon-based Belenenses football club had recently travelled to South Africa.
Each club has 10 fixtures left to play with Porto a point clear of Benfica at the top of the table.
Portugal was drawn into Group B at the World Cup, along with Morocco, Iran and Iberian rival Spain. They will be playing their first match against Spain on June 15.
CAMPINAS, Brazil : Cristiano Ronaldo is wearing a brace on his troublesome left knee as his Portugal team holds its final World Cup training session before departing for a crucial game against the United States.
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