Messi succeeded Mascherano as Argentina captain and at Barcelona, they won two Champions Leagues, two Club World Cups, and seven La Liga.
The 36-year-old centre back made the announcement after his Superliga outfit Estudiantes lost 0-1 to Argentinos Juniors.
The 36-year-old is contracted with Argentinian Superliga club Estudiantes La Plata until June 2021, however, he is unsure how his body will cope with an extended break from the game.
Mascherano, who has played at four World Cups, made his debut for Argentina in 2003 and holds the record for most appearances for Argentina with 147.
There's still a chance for Argentina to advance, but the South Americans have to win and their destiny is partly in the hands of other teams.
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