Roy suffered a laceration to his right-hand little finger during practice on Monday and will undergo a fitness test before the 3rd ODI at Leeds.
India defeated England by seven wickets to clinch the three-match series 2-1 at the County Ground, Bristol, on Sunday.
Buttler's 21-ball half-century was the fastest by an England player in this format of cricket.
England reached 314-4 in 44.4 overs in reply to 310-8 for Australia, who won the toss and chose to bat.
Roy made a mockery of a listless Mumbai bowling attack as he hammered six fours and as many sixes in his 53 ball knock to help Delhi overhaul the home team's 194/7.
Jason Roy scored a remarkable knock of 180 runs off 151 balls as England outclassed Australia by five wickets.
London: The England selectors on Sunday decided to bring back experienced pacer Tim Bresnan in the national squad for the one-off T20 International against India at the Edgbaston on September 7.The 14-member squad also features
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