The last time Barcelona advanced past the Champions League quarterfinals was in 2015, when they eventually won their fifth European title and completed the treble for the last time.
A win at Molineux would have lifted United into third place but Ole Gunnar Solskjaer's team stayed in fifth.
Liverpool beat Bayern Munich 3-1 at the Allianz Arena to secure a place in the last eight of the Champions League on Wednesday.
Lionel Messi scored two goals and assisted two more as Barcelona knocked Lyon out of the Champions League on Wednesday.
The Frenchman will not play against Olympique Lyonnais, as he is yet to recover completely from a muscle injury.
With a 7-0 thrashing of Schalke on Tuesday that completed a 10-2 aggregate victory, Manchester City eased into the last eight of Champions League.
Cristiano Ronaldo scored a hat trick against the normally resilient Spanish side to send Juventus into the Champions League quarterfinals with a 3-0 win on Tuesday.
Over the course of a week, Real Madrid lost three games at home, possibly dooming the club to end the season without a trophy.
The Spanish club is already thinking about next season, and it appears the changes will start at the top, with coach Santiago Solari unlikely to keep his job.
The Brazilian star used an Instagram post to criticize VAR officials for awarding United a decisive penalty in injury time after defender Presnel Kimpembe jumped and blocked Diogo Dalot's shot with his elbow.
Real Madrid's embarrassing 4-1 loss to Ajax on home turf on Tuesday sent the three-time defending champions out of the competition in the round of 16.
After this season ends, Ajax will likely have to go back to The Future — the name of their storied youth academy — to start rebuilding. Again.
The event, which will take place at Tote on the Turf, is a part of a series of global fan initiatives.
Frenkie De Jong, who is set to join Barcelona in the summer, called out Sergio Ramos' hubris as the Spanish defender was penalized and will have to sit out of the Tuesday match.
UEFA says its disciplinary panel found Ramos guilty of "clearly receiving a yellow card on purpose" against Ajax two weeks ago.
Diego Simeone has said he regretted the way he celebrated his team's first goal scored in Atletico Madrid's 2-0 win over Juventus in the Champions League.
Raheem Sterling scored in the 90th to earn 10-man City a 3-2 win in the first leg of its last-16 match in Gelsenkirchen.
Atletico coach Diego Simeone will be able to call on Antoine Griezmann, Diego Costa and Alvaro Morata when his team hosts Juventus in the first leg of the last 16 on Wednesday.
Pizarro, aged 40 years and 136 days, broke the previous record held by Bremen midfielder Miroslav "Mirko" Votava.
Messi took his league-leading tally to 22 goals with his 30th in all competitions this season.
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