The loss was the fourth straight for MLS in its annual All-Star game, which matches the league's top players against international competition.
The Spanish club announced the arrival of Trippier on a three-year deal Wednesday, saying he was a "fantastic and experienced" addition to the squad.
Atletico Madrid said Barcelona and Antoine Griezmann's behaviour "violates the protected periods of negotiation with players and alters the basic rules of integrity in any sporting competition.
FC Barcelona have paid Griezmann's 120 Million Euro buy-out clause and have signed the French star for a five-year contract, which has a buy-out clause of 800 Million Euros.
Atletico Madrid said it felt disrespected after learning that Barcelona and Antoine Griezmann reached an agreement in March, just days after the team was eliminated by Juventus in the Champions League.
Fernando Torres made 110 appearances for Spain, netting 38 goals and won the 2010 World Cup and the 2008 and 2012 Euro.
The left-back's contract with Atletico expires on June 30 and in recent weeks he has been linked with Barcelona and Flamengo, among other clubs.
Griezmann did not give any hints about his future but he has been widely linked with a move to Barcelona with some Spanish media saying he has already reached a deal with the Catalan club.
In clearing the path to what would be Barcelona's 26th league title, Messi reached a new personal milestone with a competition record 335th victory, overtaking Iker Casillas on 334 wins.
Cristiano Ronaldo scored a hat trick against the normally resilient Spanish side to send Juventus into the Champions League quarterfinals with a 3-0 win on Tuesday.
Barcelona came from behind to beat Rayo Vallecano 3-1 while Saul scored the sole goal of the match as Atletico Madrid beat Leganes 1-0.
fter Atletico defender Jose Maria Gimenez scored the first goal late in a 2-0 win on Wednesday, Simeone turned to his team's fans and made the gesture.
Morata netted with a left-footed shot from near the penalty spot in the 31st minute, leading second-placed Atletico to the home win and keeping it seven points from leader Barcelona.
Diego Simeone has said he regretted the way he celebrated his team's first goal scored in Atletico Madrid's 2-0 win over Juventus in the Champions League.
Ronaldo was loudly booed when his name was announced before the match, and he drew even louder jeers from the Atletico crowd when he prompted the game to be interrupted.
Atletico coach Diego Simeone will be able to call on Antoine Griezmann, Diego Costa and Alvaro Morata when his team hosts Juventus in the first leg of the last 16 on Wednesday.
The 48-year-old Simeone, who took the helm of the Spanish club in December 2011, had extended his contract on Thursday until 2022.
An overhead kick by Casemiro, a penalty kick converted by Sergio Ramos, and a second-half goal by Gareth Bale led Real Madrid to their fifth straight league win.
Atletico Madrid vs Real Madrid, Live Football Streaming: Watch Madrid derby live streaming online at SonyLiv, Live Telecast on Sony Ten 2 and Sony Ten 2 HD from Wanda Metropolitano.
The 26-year-old Spain international joined Atletico on an 18-month loan from Chelsea on Monday and was officially presented to the press on Tuesday.
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