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She signed up to the project two years ago when she saw an advert by Thomas Williams Productions looking for a virgin to film.
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Catarina previously said she wanted to plough the money $20,000 and 90 per cent of the final auction sale price into a non governmental organisation which will construct modern houses in her southern home state of Santa Catarina.
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A male virgin called Alexander, who is also being followed as part of director Justin Selsey's documentary, sold for $3,000 to a Brazilian woman called Nene B.
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Sex toys will be banned from use and a condom will be compulsory, with Catarina saying she was prepared to prove to any sceptics that she has not had sex before.
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Catarina will be 'delivered' to her buyer on board a plane between Australia and the U.S. being interviewed before and after the sexual act.
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'The auction is just business, I'm a romantic girl at heart and believe in love. But this will make a big difference to my area,' she told Folha newspaper.
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She also caused controversy when she revealed she would be followed every step of the way by an Australian crew for a documentary film called Virgins Wanted.
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The auction closed Thursday morning, and Migliorini said she will use the cash to build homes for poverty stricken familie. There were 15 bids in all for her virginity. Catarina's move sparked outrage across the globe, with many claiming she was little more than a sex worker.
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The Japanese man called Natsu, staved off strong competition from American bidders Jack Miller and Jack Right, and Indian big spender Rudra Chatterjee, to secure a date with 20 year old Catarina Migliorini.
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Migliorini claims she is not a prostitute because she is selling her body only once. She will be followed by a film crew for a documentary called Virgins Wanted.
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Catarina Migliorini, a Brazilian physical education student, has struck an online autction with a Japanese man named Natsu for $ 780,000 for selling her virginity. The Japanese man faced strong competition from an Indian man Rudra Chatterjee and two Americans.