The applicants came from over 140 countries and more than 20,000 Indians had applied for the first round.
The remaining candidates will be interviewed by the Mars One selection committee.
"We're incredibly excited to start the next phase of Round 2, where we begin to better understand our candidates who aspire to take such a daring trip. They will have to show their knowledge, intelligence, adaptability and personality," Mars One chief medical officer Norbert Kraft, said.
In December 2013, Mars One announced the selection of 1058 candidates, including 62 from India, from the original pool of over 200,000 applicants.
