TDP member M. Venugopala broke the speaker's mic.
Amidst the chaos, the house was adjourned till 2 p.m. and the house was vacated.
Rajagopal is among the six MPs from Seemandhra who were expelled from the Congress Tuesday.
The expelled MPs had said their goal is to stall tabling of the Telangana bill in parliament.
Congress expelled Sabbam Hari, G.V. Harsha Kumar, V. Arun Kumar, Lagadapati Rajagopal, R. Sambasiva Rao and A. Sai Pratap from the party for giving no-confidence motion notice against the Congress-led United Progressive Alliance (UPA) government in parliament over the Telangana issue.