Nobel Laureate poet Rabindranath Tagore, also the composer of the Indian national anthem, composed the song in 1905 against the partition of Bengal by then British rulers.
It was first sung as the country's national anthem during Bangladesh's Liberation War when the acting government was installed on April 17, 1971.
The Guinness Book officials said they would come up with a formal announcement on the record after completing the due formalities.
As part of the Independence Day celebrations, tens of thousands of Bangladeshis earlier today thronged the National Memorial at suburban Savar and offered wreaths to the martyrs of 1971.
President Abdul Hamid and the premier placed the wreaths first in a solemn ceremony when the army bugles played the last post.
Bangladesh previously formed the world's largest human national flag as it celebrated the 42nd Victory Day on December 16, 2013 marking the surrender of Pakistani troops to Indo-Bangla joint command in Dhaka after nine months of war.
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